W. Giovanni Ruiz

W. Giovanni Ruiz

: Department of Medicine

Contact Information:
University of Pittsburgh
Renal/Electrolyte Division
984 Scaife Hall
3550 Terrace Street
Pittsburgh, PA 15261

Telephone: 412-383-8892
Fax: 412-383-8955/8956
E-mail: ruizw@pitt.edu

Education and Training:

Instituto Technico Agricultura, Barcenas Villa Nueva, Guatemala, 1974-1979

Enter the name for this tabbed section: Primary Research Articles
Lavelle JP, Apodaca G, Meyers SA, Ruiz WG, Zeidel ML. Disruption of guinea pig urinary bladder permeability barrier in noninfectious cystitis. Am J Physiol. 1998 Jan;274(1 Pt 2):F205-14. PubMed PMID: 9458841.

Truschel ST, Ruiz WG, Shulman T, Pilewski J, Sun TT, Zeidel ML, Apodaca G. Primary uroepithelial cultures. A model system to analyze umbrella cell barrier function. J Biol Chem. 1999 May 21;274(21):15020-9. Review. PubMed PMID:10329705.

Leung SM, Rojas R, Maples C, Flynn C, Ruiz WG, Jou TS, Apodaca G. Modulation of endocytic traffic in polarized Madin-Darby canine kidney cells by the small GTPase RhoA. Mol Biol Cell. 1999 Dec;10(12):4369-84. PubMed PMID: 10588664; PubMed Central PMCID: PMC25764.

Jou TS, Leung SM, Fung LM, Ruiz WG, Nelson WJ, Apodaca G. Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1. Mol Biol Cell. 2000 Jan;11(1):287-304. PubMed PMID: 10637309; PubMed Central PMCID: PMC14775.

Lavelle JP, Meyers SA, Ruiz WG, Buffington CA, Zeidel ML, Apodaca G. Urothelial pathophysiological changes in feline interstitial cystitis: a human model. Am J Physiol Renal Physiol. 2000 Apr;278(4):F540-53. PubMed PMID: 10751214.

Leung SM, Ruiz WG, Apodaca G. Sorting of membrane and fluid at the apical pole of polarized Madin-Darby canine kidney cells. Mol Biol Cell. 2000 Jun;11(6):2131-50. PubMed PMID: 10848634; PubMed Central PMCID: PMC14908.

Rojas R, Ruiz WG, Leung SM, Jou TS, Apodaca G. Cdc42-dependent modulation of tight junctions and membrane protein traffic in polarized Madin-Darby canine kidney cells. Mol Biol Cell. 2001 Aug;12(8):2257-74. PubMed PMID: 11514615; PubMed Central PMCID: PMC58593.

Truschel ST, Wang E, Ruiz WG, Leung SM, Rojas R, Lavelle J, Zeidel M, Stoffer D, Apodaca G. Stretch-regulated exocytosis/endocytosis in bladder umbrella cells. Mol Biol Cell. 2002 Mar;13(3):830-46. PubMed PMID: 11907265; PubMed Central PMCID: PMC99602.

Birder LA, Nakamura Y, Kiss S, Nealen ML, Barrick S, Kanai AJ, Wang E, Ruiz G, De Groat WC, Apodaca G, Watkins S, Caterina MJ. Altered urinary bladder function in mice lacking the vanilloid receptor TRPV1. Nat Neurosci. 2002 Sep;5(9):856-60. PubMed PMID: 12161756.

Apodaca G, Kiss S, Ruiz W, Meyers S, Zeidel M, Birder L. Disruption of bladder epithelium barrier function after spinal cord injury. Am J Physiol Renal Physiol. 2003 May;284(5):F966-76. Epub 2003 Jan 14. PubMed PMID: 12527557.

Acharya P, Beckel J, Ruiz WG, Wang E, Rojas R, Birder L, Apodaca G. Distribution of the tight junction proteins ZO-1, occludin, and claudin-4, -8, and -12 in bladder epithelium. Am J Physiol Renal Physiol. 2004 Aug;287(2):F305-18. Epub 2004 Apr 6. PubMed PMID: 15068973.

Zeidel JD, Mathai JC, Campbell JD, Ruiz WG, Apodaca GL, Riordan J, Zeidel ML. Selective permeability barrier to urea in shark rectal gland. Am J Physiol Renal Physiol. 2005 Jul;289(1):F83-9. Epub 2005 Feb 22. PubMed PMID: 15727989.

Hill WG, Almasri E, Ruiz WG, Apodaca G, Zeidel ML. Water and solute permeability of rat lung caveolae: high permeabilities explained by acyl chain unsaturation. Am J Physiol Cell Physiol. 2005 Jul;289(1):C33-41. Epub 2005 Feb 23. PubMed PMID: 15728712.

Wang EC, Lee JM, Ruiz WG, Balestreire EM, von Bodungen M, Barrick S, Cockayne DA, Birder LA, Apodaca G. ATP and purinergic receptor-dependent membrane traffic in bladder umbrella cells. J Clin Invest. 2005 Sep;115(9):2412-22. Epub 2005 Aug 18. PubMed PMID: 16110327; PubMed Central PMCID: PMC1187935.

Rondanino C, Rojas R, Ruiz WG, Wang E, Hughey RP, Dunn KW, Apodaca G. RhoB-dependent modulation of postendocytic traffic in polarized Madin-Darby canine kidney cells. Traffic. 2007 Jul;8(7):932-49. Epub 2007 Jun 4. PubMed PMID: 17547697.

Khandelwal P, Ruiz WG, Balestreire-Hawryluk E, Weisz OA, Goldenring JR, Apodaca G. Rab11a-dependent exocytosis of discoidal/fusiform vesicles in bladder umbrella cells. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15773-8. Epub 2008 Oct 8. PubMed PMID: 18843107; PubMed Central PMCID: PMC2572972.

Khandelwal P, Ruiz WG, Apodaca G. Compensatory endocytosis in bladder umbrella cells occurs through an integrin-regulated and RhoA- and dynamin-dependent pathway. EMBO J. 2010 Jun 16;29(12):1961-75. Epub 2010 May 11. PubMed PMID:20461056.

Yu W, Hill WG, Apodaca G, Zeidel ML. Expression and distribution of transient receptor potential (TRP) channels in bladder epithelium. Am J Physiol Renal Physiol. 2011 Jan;300(1):F49-59. Epub 2010 Oct 13. PubMed PMID: 20943764; PubMed Central PMCID: PMC3023226.

Rondanino C, Poland PA, Kinlough CL, Li H, Rbaibi Y, Myerburg MM, Al-bataineh MM, Kashlan OB, Pastor-Soler NM, Hallows KR, Weisz OA, Apodaca G, Hughey RP. Galectin-7 modulates the length of the primary cilia and wound repair in polarized kidney epithelial cells. Am J Physiol Renal Physiol. 2011 Sep;301(3):F622-33. Epub 2011 Jun 15. PubMed PMID: 21677144; PubMed Central PMCID: PMC3174547.

Mitra S, Lukianov S, Ruiz WG, Cianciolo Cosentino C, Sanker S, Traub LM, Hukriede NA, Apodaca G. Requirement for a uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity. PLoS One. 2012;7(7):e41816. Epub 2012 Jul 25. PubMed PMID: 22848617; PubMed Central PMCID: PMC3404999.

Hanna-Mitchell AT, Ruiz GW, Daneshgari F, Liu G, Apodaca G, Birder LA. Impact of diabetes mellitus on bladder uroepithelial cells. Am J Physiol Regul Integr Comp Physiol. 2013 Jan 15;304(2):R84-93. doi: 10.1152/ajpregu.00129.2012. Epub 2012 Nov 21. PubMed PMID: 23174855; PubMed Central PMCID: PMC3543662.

Szalinski CM, Guerriero CJ, Ruiz WG, Docter BE, Rbaibi Y, Pastor-Soler NM, Apodaca G, Puthenveedu MA, Weisz OA. PIP5KIβ Selectively Modulates Apical Endocytosis in Polarized Renal Epithelial Cells. PLoS One. 2013;8(1):e53790. PubMed PMID: 23342003; PubMedCentral PMCID: PMC3547069.

Khandelwal P, Prakasam HS, Clayton DR, Ruiz WG, Gallo LI, van Roekel D, Lukianov S, Peränen J, Goldenring JR, Apodaca G. A Rab11a-Rab8a-Myo5B network promotes stretch-regulated exocytosis in bladder umbrella cells. Mol Biol Cell. 2013 Apr;24(7):1007-19. Epub 2013 Feb 6. PubMed Central PMCID: PMC3608489.

Carattino MD, Prakasam HS, Ruiz WG, Clayton DR, McGuire M, Gallo LI, Apodaca G. Bladder filling and voiding affect umbrella cell tight junction organization and function. Am J Physiol Renal Physiol. 2013 Oct;305(8):F1158-F1168. Epub 2013 Jul 24. PubMed PMID: 23884145.

Gallo LI, Liao Y, Ruiz WG, Clayton DR, Li M, Liu Y-J, Jiang Y, Fukuda M, Apodaca G, Yin X-M. TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells. Mol. Biol. Cell 2014 (in press)

Prakasam HS, Gallo LI, Li H, Ruiz WG, Hallows KR, Apodaca G. A1 adenosine receptor-stimulated exocytosis in bladder umbrella cells requires phosphorylation of ADAM17 Ser-811 and EGF receptor transactivation. Mol Biol Cell. 2014 Nov 15;25(23):3798-812. doi: 10.1091/mbc.E14-03-0818. Epub 2014 Sep 17. PubMed PMID: 25232008; PubMed Central PMCID: PMC4230785.

Montalbetti N, Rued AC, Clayton DR, Ruiz WG, Bastacky SI, Prakasam HS, Eaton AF, Kullmann FA, Apodaca G, Carattino MD. Increased urothelial paracellular transport promotes cystitis. Am J Physiol Renal Physiol. 2015 Dec 15;309(12):F1070-81. doi: 10.1152/ajprenal.00200.2015. Epub 2015 Sep 30. PubMed PMID: 26423859; PubMed Central PMCID: PMC4683306.

Sneddon WB, Ruiz GW, Gallo LI, Xiao K, Zhang Q, Rbaibi Y, Weisz OA, Apodaca GL, Friedman PA. Convergent Signaling Pathways Regulate Parathyroid Hormone and Fibroblast Growth Factor-23 Action on NPT2A-mediated Phosphate Transport. J Biol Chem. 2016 Jul 18. pii: jbc.M116.744052. [Epub ahead of print] PubMed PMID: 27432882.

Enter the name for this tabbed section: Review Articles
Enter the name for this tabbed section: Books and Chapters