Apodaca Lab Environment

Stacks Image 712
The City of Pittsburgh:

The Pittsburgh metropolitan area population is 2.4 million, although only 300,000 residents live within the city itself. The city was once the center of the steel industry; however, it has now shed its outdated industrial image and is gaining recognition worldwide for its advanced medical facilities, major universities, myriad cultural institutions, and start-up technology companies. Pittsburgh has one of the countries most dynamic visual and performing arts and music scene, and the city contains appealing vistas, large parks and recreational facilities, and beautiful neighborhoods. Pittsburgh has been rated as the nation’s “most livable” city by Places Rated Almanac, Forbes and The Economist. Use the "Links" page to learn more about the city and surrounding communities

The University:

The University of Pittsburgh is and urban campus located in the Oakland District of the city of Pittsburgh. It is in the top cluster of U.S. public research universities and ranks 5th among all research universities in competitive grants awarded to its faculty by the National Institutes of Health. The University has a large and growing number of world-class science programs covering a broad spectrum of disciplines including: molecular biology, cellular biology, developmental biology, structural biology, drug discovery and design, immunology, neuroscience, organ and tissue engineering, regenerative medicine, cancer diagnostics and treatment, cardiology, gene therapy, bioinformatics and computational biology, nanoscience, and medical device development. The University also has numerous research centers that support cell and molecular biology research at the University. Use the "Links" tab above to learn more about these centers.

The Apodaca Laboratory:

The Apodaca laboratory is housed in the University of Pittsburgh School of Medicine, Department of Medicine, Renal-Electrolyte Division. The laboratory is located in rooms 982 and 984 of Scaife Hall on the main campus of the University (click here to see our lab location and to get a map of the campus). We are one of 11 basic-science laboratories in our Division and also maintain close ties with laboratories in the Department of Cell Biology & Physiology and the Departments of Biological Sciences at the University of Pittsburgh and the Carnegie Mellon University. The Division is home to the NIH-sponsored George M. O'Brien Pittsburgh Center for Kidney Research, one of nine such centers in the nation. Dr. Apodaca is director of the center's Urinary Tract Epithelial Imaging Core.

Equipment and Facilities:

The lab has numerous computer workstations: each bench is equipped with an Intel-based Macintosh computer, there is an 8-core MacPro running Perkin-Elmer Volocity software, and the lab has a server cluster with a RAID storage device. Color and B&W printers are available as are several word processing, graphics, and image analysis software packages. The laboratory has all of the equipment necessary to perform protein and DNA biochemistry including electrophoresis equipment and PCR machines. Moreover, we have several dedicated pressure chamber/capacitance systems to perform our studies of membrane traffic in bladder epithelium. The lab tissue culture facility includes two hoods, four incubators, and a light microscope. In addition, the adjacent Imaging Core houses our JEOL TS100 scanning electron microscope, our JEOL 100CX transmission electron microscope, and equipment for sample preparation including two Leica ultramicrotomes/cryoultramicrotomes, a Leica cryotome, a Tusimis critical point dryer, a sputter coater, and a Leica knifemaker. Light microscopes include a Nikon Optiphot widefield microscope equipped for phase/contrast and fluorescence microscopy, a Leica DM6000 epifluorescence/DIC microscope equipped with a QImaging digital camera and Volocity Acquisition software, and a Leica M125 stereomicroscope with fluorescence and QImaging camera. In addition, we have a Perkin-Elmer spinning disk confocal system and a Leica CW-STED super-resolution confocal microscope. Other divisional facilities include an HPLC/FPLC set-up for protein purification, high speed and ultra centrifuges, gamma and scintillation counters, and a BioRad phosphorimager and Versadoc.