Research Specialist IV
Department of Medicine
Contact Information:
University of Pittsburgh, Renal/Electrolyte Division, 984 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA 15261
Telephone: 412-648-9105, Fax: 412-383-8955/8956, E-mail: ruizw@pitt.edu
Education and Training:
Undergraduate:
Instituto Technico Agricultura, Barcenas Villa Nueva, Guatemala, 1974-1979
Publications (in chronological order):
Maples CJ, Ruiz WG, Apodaca G. Both microtubules and actin filaments are required for efficient postendocytotic traffic of the polymeric immunoglobulin receptor in polarized Madin-Darby canine kidney cells. J Biol Chem. 1997 Mar 7;272(10):6741-51. PubMed PMID: 9045707.
Lavelle JP, Apodaca G, Meyers SA, Ruiz WG, Zeidel ML. Disruption of guinea pig urinary bladder permeability barrier in noninfectious cystitis. Am J Physiol. 1998 Jan;274(1 Pt 2):F205-14. PubMed PMID: 9458841.
Truschel ST, Ruiz WG, Shulman T, Pilewski J, Sun TT, Zeidel ML, Apodaca G. Primary uroepithelial cultures. A model system to analyze umbrella cell barrier function. J Biol Chem. 1999 May 21;274(21):15020-9. Review. PubMed PMID:10329705.
Altschuler Y, Liu S, Katz L, Tang K, Hardy S, Brodsky F, Apodaca G, Mostov K. ADP-ribosylation factor 6 and endocytosis at the apical surface of Madin-Darby canine kidney cells. J Cell Biol. 1999 Oct 4;147(1):7-12. PubMed PMID: 10508850; PubMed Central PMCID: PMC2164974.
Leung SM, Rojas R, Maples C, Flynn C, Ruiz WG, Jou TS, Apodaca G. Modulation of endocytic traffic in polarized Madin-Darby canine kidney cells by the small GTPase RhoA. Mol Biol Cell. 1999 Dec;10(12):4369-84. PubMed PMID: 10588664; PubMed Central PMCID: PMC25764.
Jou TS, Leung SM, Fung LM, Ruiz WG, Nelson WJ, Apodaca G. Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1. Mol Biol Cell. 2000 Jan;11(1):287-304. PubMed PMID: 10637309; PubMed Central PMCID: PMC14775.
Lavelle JP, Meyers SA, Ruiz WG, Buffington CA, Zeidel ML, Apodaca G. Urothelial pathophysiological changes in feline interstitial cystitis: a human model. Am J Physiol Renal Physiol. 2000 Apr;278(4):F540-53. PubMed PMID: 10751214.
Leung SM, Ruiz WG, Apodaca G. Sorting of membrane and fluid at the apical pole of polarized Madin-Darby canine kidney cells. Mol Biol Cell. 2000 Jun;11(6):2131-50. PubMed PMID: 10848634; PubMed Central PMCID: PMC14908.
Rojas R, Ruiz WG, Leung SM, Jou TS, Apodaca G. Cdc42-dependent modulation of tight junctions and membrane protein traffic in polarized Madin-Darby canine kidney cells. Mol Biol Cell. 2001 Aug;12(8):2257-74. PubMed PMID: 11514615; PubMed Central PMCID: PMC58593.
Truschel ST, Wang E, Ruiz WG, Leung SM, Rojas R, Lavelle J, Zeidel M, Stoffer D, Apodaca G. Stretch-regulated exocytosis/endocytosis in bladder umbrella cells. Mol Biol Cell. 2002 Mar;13(3):830-46. PubMed PMID: 11907265; PubMed Central PMCID: PMC99602.
Apodaca G, Kiss S, Ruiz W, Meyers S, Zeidel M, Birder L. Disruption of bladder epithelium barrier function after spinal cord injury. Am J Physiol Renal Physiol. 2003 May;284(5):F966-76. Epub 2003 Jan 14. PubMed PMID: 12527557.
Acharya P, Beckel J, Ruiz WG, Wang E, Rojas R, Birder L, Apodaca G. Distribution of the tight junction proteins ZO-1, occludin, and claudin-4, -8, and -12 in bladder epithelium. Am J Physiol Renal Physiol. 2004 Aug;287(2):F305-18. Epub 2004 Apr 6. PubMed PMID: 15068973.
Hill WG, Almasri E, Ruiz WG, Apodaca G, Zeidel ML. Water and solute permeability of rat lung caveolae: high permeabilities explained by acyl chain unsaturation. Am J Physiol Cell Physiol. 2005 Jul;289(1):C33-41. Epub 2005 Feb 23. PubMed PMID: 15728712.
Wang EC, Lee JM, Ruiz WG, Balestreire EM, von Bodungen M, Barrick S, Cockayne DA, Birder LA, Apodaca G. ATP and purinergic receptor-dependent membrane traffic in bladder umbrella cells. J Clin Invest. 2005 Sep;115(9):2412-22. Epub 2005 Aug 18. PubMed PMID: 16110327; PubMed Central PMCID: PMC1187935.
Rondanino C, Rojas R, Ruiz WG, Wang E, Hughey RP, Dunn KW, Apodaca G. RhoB-dependent modulation of postendocytic traffic in polarized Madin-Darby canine kidney cells. Traffic. 2007 Jul;8(7):932-49. Epub 2007 Jun 4. PubMed PMID: 17547697.
Khandelwal P, Ruiz WG, Balestreire-Hawryluk E, Weisz OA, Goldenring JR, Apodaca G. Rab11a-dependent exocytosis of discoidal/fusiform vesicles in bladder umbrella cells. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15773-8. Epub 2008 Oct 8. PubMed PMID: 18843107; PubMed Central PMCID: PMC2572972.
Khandelwal P, Ruiz WG, Apodaca G. Compensatory endocytosis in bladder umbrella cells occurs through an integrin-regulated and RhoA- and dynamin-dependent pathway. EMBO J. 2010 Jun 16;29(12):1961-75. Epub 2010 May 11. PubMed PMID:20461056.